What amino acid residues are detected in bradford essay

Due to the aromatic amino acid residues in the protein, the observed UV absorbance was mainly in the nm to nm region. In Figure 1 to 3, the maximal absorbance of each protein was approximately at nm. The difference in magnitudes of the peak observed was linked to the differences in the amino acid contents in each of the proteins. The peak of lysozyme was greater than those of BSA and gelatin, because lysozyme.
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The Bradford Protein Assay

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Bradford Assay On Unknown Concentrations Of Proteins | Bartleby

The purpose of this lab is to determine if the Bradford assay is an accurate way to determine an unknown concentration of two samples of protein. The Bradford assay is done by measuring wavelength of light passing. Due to the aromatic amino acid residues in the protein, the observed UV absorbance was mainly in the nm to nm region. In Figure 1 to 3, the maximal absorbance of each protein was approximately at nm. The difference in magnitudes of the peak observed was linked to the differences in the amino acid contents in each of the proteins.
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Bradford protein assay

Please join StudyMode to read the full document. The more diluted the solution was, the less blue it was. The R2 value of the standard curve of BSA dilution was obtained to be 0. The closer to 1 the R2 value was, the more accurate the linear portion was.
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Please join StudyMode to read the full document. The more diluted the solution was, the less blue it was. The R2 value of the standard curve of BSA dilution was obtained to be 0.
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